Journal:

Article Title: The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities

doi: 10.1016/j.biomaterials.2008.01.035

Figure Lengend Snippet: Measurement of active MMP-13 produced by hMSCs encapsulated in plain PEG gels and cultured in either control stem cell media (□) or chondrogenic differentiation media (■). MMP-13 production was normalized to DNA content to better quantify MMP production per cell basis.

Article Snippet: Active MMP-13 enzyme production levels were monitored over a two week time course via a FRET modified ELISA technique based upon a MMP-13 specific kit (AnaSpec).

Techniques: Produced, Cell Culture

Journal:

Article Title: The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities

doi: 10.1016/j.biomaterials.2008.01.035

Figure Lengend Snippet: Characterization of peptide release from PEG gels. Varying concentrations of tryptophan containing peptide sequence were copolymerized with PEG and treated with 0, 10, 50, 100 and 500ng/ml exogenously delivered MMP-13. PEG-peptide concentrations were as follows; 10mM (■), 5mM (●), 0mM (□), 10mM scramble sequence (▲). UV-Vis measurements for released tryptophan residue (A) were normalized to loaded concentrations to determine a release profile. (B) RGD ELISA was performed on 10mM peptide containing PEG gels treated with either 0 or 500ng/ml exogenously delivered MMP-13. Surface concentration determining RGD release was fit to a standard curve of known RGD concentrations.

Article Snippet: Active MMP-13 enzyme production levels were monitored over a two week time course via a FRET modified ELISA technique based upon a MMP-13 specific kit (AnaSpec).

Techniques: Sequencing, Enzyme-linked Immunosorbent Assay, Concentration Assay